Recombinant human METTL1 protein
METTL1 is known as tRNA (guanine-N (7) -) -methyltransferase which belongs to the methyltransferase superfamily. METTL1 shows high sequence similarity to yeast ORF YDL201w and has been shown to be inactivated by phosphorylation. This protein contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphylation. It catalyzes the formation of N (7) -methylguanine at position 46 (m7G46) in tRNA. Recombinant human METTL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Methyltransferase like 1, TRM8, YDL201w
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
33.6 kDa (296aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Matsumoto K., et al. (2008) J. Biotechnol. 133: 453-460.; ; Alexandrov A., et al. (2002) RNA 8:1253-1266;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_005362
Uniprot Accession Number
Q9UBP6
Species
Human
AA Sequence
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