Recombinant human METTL1 protein

METTL1 is known as tRNA (guanine-N (7) -) -methyltransferase which belongs to the methyltransferase superfamily. METTL1 shows high sequence similarity to yeast ORF YDL201w and has been shown to be inactivated by phosphorylation. This protein contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphylation. It catalyzes the formation of N (7) -methylguanine at position 46 (m7G46) in tRNA. Recombinant human METTL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Methyltransferase like 1, TRM8, YDL201w

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33.6 kDa (296aa) confirmed by MALDI-TOF

Additionnal Information

METTL1, Methyltransferase like 1, TRM8, YDL201w, ATGP0869-10 µg, ATGP0869-20 µg, ATGP0869-50 µg, ATGP0869-100 µg, ATGP0869-250 µg, ATGP0869-500 µg, ATGP0869-1 mg, ATGP0869-10, ATGP0869-20, ATGP0869-50, ATGP0869-100, ATGP0869-250, ATGP0869-500, ATGP0869-1

References & Citations

Matsumoto K., et al. (2008) J. Biotechnol. 133: 453-460.; ; Alexandrov A., et al. (2002) RNA 8:1253-1266;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_005362

Uniprot Accession Number

Q9UBP6

Species

Human

AA Sequence

MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK NFPAIFRRIQ DPVLQAVTSQ TSLPGH