Recombinant human NHP2 protein
NHP2, also called NOLA2, forms a small ribonucleoprotein particle with GAR1 (NOLA1), Nop10 for the isomerization of uridine to pseudouridine. This protein is involved in various aspects of rRNA processing and modification. It localizes to the dense fibrillar component of the nucleolus and in nuclear Cajal bodies. Recombinant human NHP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
H/ACA ribonucleoprotein complex subunit 2 isoform a, NHP2P, NOLA2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
19.3 kDa (173aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Henras A., et al. (1998), EMBO J., 17 (23) :7078-90.; ; Pogacic V., et al. (2000) . Mol. Cell. Biol.: 20:9028-9040;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_060308
Uniprot Accession Number
Q9NX24
Species
Human
AA Sequence
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