Recombinant human NHP2 protein

NHP2, also called NOLA2, forms a small ribonucleoprotein particle with GAR1 (NOLA1), Nop10 for the isomerization of uridine to pseudouridine. This protein is involved in various aspects of rRNA processing and modification. It localizes to the dense fibrillar component of the nucleolus and in nuclear Cajal bodies. Recombinant human NHP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

H/ACA ribonucleoprotein complex subunit 2 isoform a, NHP2P, NOLA2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.3 kDa (173aa) confirmed by MALDI-TOF

Additionnal Information

NHP2, H/ACA ribonucleoprotein complex subunit 2 isoform a, NHP2P, NOLA2, ATGP0865-10 µg, ATGP0865-20 µg, ATGP0865-50 µg, ATGP0865-100 µg, ATGP0865-250 µg, ATGP0865-500 µg, ATGP0865-1 mg, ATGP0865-10, ATGP0865-20, ATGP0865-50, ATGP0865-100, ATGP0865-250, ATGP0865-500, ATGP0865-1

References & Citations

Henras A., et al. (1998), EMBO J., 17 (23) :7078-90.; ; Pogacic V., et al. (2000) . Mol. Cell. Biol.: 20:9028-9040;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_060308

Uniprot Accession Number

Q9NX24

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MTKIKADPDG PEAQAEACSG ERTYQELLVN QNPIAQPLAS RRLTRKLYKC IKKAVKQKQI RRGVKEVQKF VNKGEKGIMV LAGDTLPIEV YCHLPVMCED RNLPYVYIPS KTDLGAAAGS KRPTCVIMVK PHEEYQEAYD ECLEEVQSLP LPL