Recombinant human Calcineurin A/PPP3CA protein

PPP3CA, also known as Calcineurin A, is a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. It activates the T cells of the immune system and can be blocked by drugs. PPP3CA activates NFATc, a transcription factor by dephosphorylating it. The activated NFATc is then translocated into the nucleus, where it upregulates the expression of interleukin 2. Recombinant human PPP3CA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein phosphatase 3 catalytic subunit alpha, CAM-PRP catalytic subunit, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CNA alpha, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CALNA, CALN, CNA, CNA1, PPP2B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

60 kDa (534aa)

Additionnal Information

PPP3CA, Serine/threonine-protein phosphatase 2B catalytic subunit alpha, CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B, ATGP0861-10 µg, ATGP0861-20 µg, ATGP0861-50 µg, ATGP0861-100 µg, ATGP0861-250 µg, ATGP0861-500 µg, ATGP0861-1 mg, ATGP0861-10, ATGP0861-20, ATGP0861-50, ATGP0861-100, ATGP0861-250, ATGP0861-500, ATGP0861-1

References & Citations

Ostenfeld MS., et al. (2010) Oncogene. 29 (7) :1073-84.; ; Rodriguez A., et al. (2009) Mol Cell. 33 (5) :616-26.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001124163

Uniprot Accession Number

Q08209

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH TGSMSEPKAI DPKLSTTDRV VKAVPFPPSH RLTAKEVFDN DGKPRVDILK AHLMKEGRLE ESVALRIITE GASILRQEKN LLDIDAPVTV CGDIHGQFFD LMKLFEVGGS PANTRYLFLG DYVDRGYFSI ECVLYLWALK ILYPKTLFLL RGNHECRHLT EYFTFKQECK IKYSERVYDA CMDAFDCLPL AALMNQQFLC VHGGLSPEIN TLDDIRKLDR FKEPPAYGPM CDILWSDPLE DFGNEKTQEH FTHNTVRGCS YFYSYPAVCE FLQHNNLLSI LRAHEAQDAG YRMYRKSQTT GFPSLITIFS APNYLDVYNN KAAVLKYENN VMNIRQFNCS PHPYWLPNFM DVFTWSLPFV GEKVTEMLVN VLNICSDDEL GSEEDGFDGA TAAARKEVIR NKIRAIGKMA RVFSVLREES ESVLTLKGLT PTGMLPSGVL SGGKQTLQSA IKGFSPQHKI TSFEEAKGLD RINERMPPRR DAMPSDANLN SINKALTSET NGTDSNGSNS SNIQ