Recombinant human LSM4 protein
LSM4, also called u6 snRNA-associated Sm-like protein or glycine-rich protein (GRP), forms donut-shaped heptameric complexes that are involved in various steps of RNA metabolism. It facilitates RNA protein interactions and structural changes that are required during ribosomal subunit assembly. It binds specifically to the 3'-terminal u-tract of u6 snRNA. Recombinant human LSM4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
U6 snRNA-associated Sm-like protein LSm4, YER112W
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
17.5 kDa (159aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hirsch E., et al. (2000), Mol. Cell. Biol., 20: 1055-1062.; ; Brahms H.., et al. (2001) . RNA., 7: 1531-1542;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_036453
Uniprot Accession Number
Q9Y4Z0
Species
Human
AA Sequence
Frequently Asked Questions
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