Recombinant human Coactosin-like Protein 1/COTL1 protein
COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. COTL1 Binds to F-actin in a calcium-independent manner and has no direct effect on actin depolymerization. Recombinant human COTL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Coactosin like F-actin binding protein 1, Coactosin-like 1 (Dictyostelium), Coactosin-like F-actin binding protein 1, CLP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
18.1 kDa (162aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Rakonjac M., et al. (2006) Proc Natl Acad Sci u S A. 103 (35) :13150-5. ; ; Provost P., et al. (2001) Biochem J. 359 (2) :255-63.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_066972.1
Uniprot Accession Number
Q14019
Species
Human
AA Sequence
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