Recombinant human CRKL protein
CRKL is a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JuN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substitutete of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and has oncogenic potential. Recombinant human CRKL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
CRK like proto-oncogene, Adaptor protein, V-crk avian sarcoma virus CT10 oncogene homolog-like
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35.9 kDa (323aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Arai A., et al. (2001) J Biol Chem. 276 (35) :33282-90. ; ; Senechal K., et al. (1996) J Biol Chem. 271 (38) :23255-61.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Scientific Category
Cancer
NCBI Accession Number
NP_005198.1
Uniprot Accession Number
P46109
Species
Human
AA Sequence
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