Recombinant human CRKL protein

CRKL is a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JuN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substitutete of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and has oncogenic potential. Recombinant human CRKL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CRK like proto-oncogene, Adaptor protein, V-crk avian sarcoma virus CT10 oncogene homolog-like

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.9 kDa (323aa) confirmed by MALDI-TOF

Additionnal Information

CrkL, Crk-like protein, ATGP0857-10 µg, ATGP0857-20 µg, ATGP0857-50 µg, ATGP0857-100 µg, ATGP0857-250 µg, ATGP0857-500 µg, ATGP0857-1 mg, ATGP0857-10, ATGP0857-20, ATGP0857-50, ATGP0857-100, ATGP0857-250, ATGP0857-500, ATGP0857-1

References & Citations

Arai A., et al. (2001) J Biol Chem. 276 (35) :33282-90. ; ; Senechal K., et al. (1996) J Biol Chem. 271 (38) :23255-61.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Cancer

NCBI Accession Number

NP_005198.1

Uniprot Accession Number

P46109

Species

Human

AA Sequence

MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE