Recombinant human WHSC2/NELFA protein

WHSC2, also known as NELF-A, is a protein factor required for DRB-sensitive transcription. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16. 3) . Recombinant human WHSC2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Wolf-Hirschhorn syndrome candidate 2, NELF-A, NELFA, P/OKcl.15, Negative elongation factor A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

60.6 kDa (559aa)

Additionnal Information

WHSC2, Wolf-Hirschhorn syndrome candidate 2, NELF-A, NELFA, P/OKcl.15, Negative elongation factor A, ATGP0856-10 µg, ATGP0856-20 µg, ATGP0856-50 µg, ATGP0856-100 µg, ATGP0856-250 µg, ATGP0856-500 µg, ATGP0856-1 mg, ATGP0856-10, ATGP0856-20, ATGP0856-50, ATGP0856-100, ATGP0856-250, ATGP0856-500, ATGP0856-1

References & Citations

Care A., et al. (2007) Nat Med. 13 (5) :613-8.; ; Mariotti M., et al. (2000) FEBS Lett. 487 (2) :166-70.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005654

Uniprot Accession Number

Q9H3P2

Species

Human

AA Sequence

MPGQRRALSP KMASMRESDT GLWLHNKLGA TDELWAPPSI ASLLTAAVID NIRLCFHGLS SAVKLKLLLG TLHLPRRTVD EMKGALMEII QLASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP VKHFQLKRKP KSATLRAELL QKSTETAQQL KRSAGVPFHA KGRGLLRKMD TTTPLKGIPK QAPFRSPTAP SVFSPTGNRT PIPPSRTLLR KERGVKLLDI SELDMVGAGR EAKRRRKTLD AEVVEKPAKE ETVVENATPD YAAGLVSTQK LGSLNNEPAL PSTSYLPSTP SVVPASSYIP SSETPPAPSS REASRPPEEP SAPSPTLPAQ FKQRAPMYNS GLSPATPTPA APTSPLTPTT PPAVAPTTQT PPVAMVAPQT QAPAQQQPKK NLSLTREQMF AAQEMFKTAN KVTRPEKALI LGFMAGSREN PCQEQGDVIQ IKLSEHTEDL PKADGQGSTT MLVDTVFEMN YATGQWTRFK KYKPMTNVS