Recombinant human Rap2A protein

RAP2A, also known as RAP2, is a member of RAS oncogene family and a Ras-like small G protein. This protein shares 60% identity with Rap1A and exhibits a carboxy-terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap1A and Rap1B, overexpression of RAP2A does not interfere with the Ras signaling pathway. Recombinant human RAP2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Rap-2a, K-REV, KREV, RAP2, RbBP-30

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.4 kDa (200aa) confirmed by MALDI-TOF

Additionnal Information

Rap2A, Ras-related protein Rap-2a, K-REV, KREV, RAP2, RbBP-30, ATGP0855-10 µg, ATGP0855-20 µg, ATGP0855-50 µg, ATGP0855-100 µg, ATGP0855-250 µg, ATGP0855-500 µg, ATGP0855-1 mg, ATGP0855-10, ATGP0855-20, ATGP0855-50, ATGP0855-100, ATGP0855-250, ATGP0855-500, ATGP0855-1

References & Citations

Yaman E., et al. (2009) FEBS J. 276 (16) :4607-16.; ; Das R., et al. (2008) J Biol Chem. 283 (28) :19691-703.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_066361

Uniprot Accession Number

P10114

Species

Human

AA Sequence

MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYEKVP VILVGNKVDL ESEREVSSSE GRALAEEWGC PFMETSAKSK TMVDELFAEI VRQMNYAAQP DKDDPCCSAC