Recombinant human MAPRE1 protein
MAPRE1, also known as EB1, belongs to the intermediate/early gene family. This protein first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. It localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. MAPRE1 is involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. Recombinant human MAPRE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Microtubule-associated protein RP/EB family member 1., Microtubule-associated protein RP/EB family member 1., EB1, MGC117374, MGC129946
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
32.1 kDa (288aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhang T., et al. (2009) J Cell Sci. 122 (Pt 9) :1401-9. ; ; Tirnauer JS., et al. (2002) Mol Biol Cell. 13 (10) :3614-26.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_036457
Uniprot Accession Number
Q15691
Species
Human
AA Sequence
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