Recombinant human PSMB5 protein
PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit) . PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway. Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Proteasome (prosome macropain) subunit beta type 5., LMPX, MB1, MGC104214, X.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.7 kDa (241aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Coux O., et al. (1996) Annu Rev Biochem. 65:801-47. ; ; Wenzel T., et al. (1995) Nat Struct Biol. 2 (3) :199-204.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 50% glycerol, 1mM DTT, 0.15M NaCl
Scientific Category
Cell Cycle
NCBI Accession Number
NP_002788
Uniprot Accession Number
P28074
Species
Human
AA Sequence
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