Recombinant human PSMB5 protein

PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit) . PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway. Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome (prosome macropain) subunit beta type 5., LMPX, MB1, MGC104214, X.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.7 kDa (241aa) confirmed by MALDI-TOF

Additionnal Information

PSMB5, Proteasome (prosome macropain) subunit beta type 5., LMPX, MB1, MGC104214, X., ATGP0850-10 µg, ATGP0850-20 µg, ATGP0850-50 µg, ATGP0850-100 µg, ATGP0850-250 µg, ATGP0850-500 µg, ATGP0850-1 mg, ATGP0850-10, ATGP0850-20, ATGP0850-50, ATGP0850-100, ATGP0850-250, ATGP0850-500, ATGP0850-1

References & Citations

Coux O., et al. (1996) Annu Rev Biochem. 65:801-47. ; ; Wenzel T., et al. (1995) Nat Struct Biol. 2 (3) :199-204.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 50% glycerol, 1mM DTT, 0.15M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002788

Uniprot Accession Number

P28074

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P