Recombinant human RhoA protein
RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila) . Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ras homolog gene family member A, ARH12, ARHA, RHO12, RHOH12, Transforming protein RhoA, Rho cDNA clone 12
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.7 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Madaule P. et al. (1985) Cell. 41:31-40; ; Garrett M.D. et al. (1989) J. Biol. Chem. 264:10-13
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_001655.1
Uniprot Accession Number
P61586
Species
Human
AA Sequence
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