Recombinant human RhoA protein

RhoA belongs to the small GTPase superfamily, Rho family, proteins involved in the regulation and timing of cell division. This protein is a small GTPase protein to regulate the actin cytoskeleton in the formation of stress fibers and acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila) . Recombinant human RhoA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras homolog gene family member A, ARH12, ARHA, RHO12, RHOH12, Transforming protein RhoA, Rho cDNA clone 12

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.7 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Ras homolog gene family, member A, Ras homolog gene family member A, ARH12, ARHA, RHO12, RHOH12, Transforming protein RhoA, Rho cDNA clone 12, RHOA, ATGP0849-10 µg, ATGP0849-20 µg, ATGP0849-50 µg, ATGP0849-100 µg, ATGP0849-250 µg, ATGP0849-500 µg, ATGP0849-1 mg, ATGP0849-010, ATGP0849-020, ATGP0849-050, ATGP0849-100, ATGP0849-250, ATGP0849-500, ATGP0849-01M

References & Citations

Madaule P. et al. (1985) Cell. 41:31-40; ; Garrett M.D. et al. (1989) J. Biol. Chem. 264:10-13

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_001655.1

Uniprot Accession Number

P61586

Species

Human

AA Sequence

MAA IRKKLVIVGD GACGKTCLLI VFSKDQFPEV YVPTVFENYV ADIEVDGKQV ELALWDTAGQ EDYDRLRPLS YPDTDVILMC FSIDSPDSLE NIPEKWTPEV KHFCPNVPII LVGNKKDLRN DEHTRRELAK MKQEPVKPEE GRDMANRIGA FGYMECSAKT KDGVREVFEM ATRAALQARR GKKKSGC