Recombinant human ATP5O protein

ATP synthase subunit O, also known as ATP5O, localizes to the mitochondria and catalyzes ATP synthesis. The protein is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance. Recombinant human ATP5O protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ATP synthase subunit O, ATPO, OSCP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.1 kDa (211aa) confirmed by MALDI-TOF

Additionnal Information

ATP5O, ATP synthase subunit O, ATPO, OSCP, ATGP0847-10 µg, ATGP0847-20 µg, ATGP0847-50 µg, ATGP0847-100 µg, ATGP0847-250 µg, ATGP0847-500 µg, ATGP0847-1 mg, ATGP0847-10, ATGP0847-20, ATGP0847-50, ATGP0847-100, ATGP0847-250, ATGP0847-500, ATGP0847-1

References & Citations

Hundal T., et al. (1984) J Bioenerq Biomembr. 16:535-550.; ; Joshi S., et al. (1992) J Biol Chem. 267:12860-12867.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001688

Uniprot Accession Number

P48047

Species

Human

AA Sequence

FAKLVRPPV QVYGIEGRYA TALYSAASKQ NKLEQVEKEL LRVAQILKEP KVAASVLNPY VKRSIKVKSL NDITAKERFS PLTTNLINLL AENGRLSNTQ GVVSAFSTMM SVHRGEVPCT VTSASPLEEA TLSELKTVLK SFLSQGQVLK LEAKTDPSIL GGMIVRIGEK YVDMSVKTKI QKLGRAMREI V