Recombinant human RAB5B protein

Ras-related protein Rab-5B, also known as RAB5B, is a member of the Rab family of small (monomeric) G proteins. RAB5B is involved in endocytosis and recycling of cell surface molecules. It interacts with RIN2 and RIN3, which regulate its function, possibly by acting as GEFs. Knockdown of RAB5B abolished group I metabotropic glutamate receptor (mGluR) -mediated neuroprotection. Recombinant human RAB5B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Rab-5B, Rab 5b, Rab5 b, RAB5B member RAS oncogene family, Ras related protein Rab 5B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.1 kDa (238aa) confirmed by MALDI-TOF

Additionnal Information

RAB5B, Ras-related protein Rab-5B, Rab 5b, Rab5 b, RAB5B member RAS oncogene family, Ras related protein Rab 5B, ATGP0846-10 µg, ATGP0846-20 µg, ATGP0846-50 µg, ATGP0846-100 µg, ATGP0846-250 µg, ATGP0846-500 µg, ATGP0846-1 mg, ATGP0846-10, ATGP0846-20, ATGP0846-50, ATGP0846-100, ATGP0846-250, ATGP0846-500, ATGP0846-1

References & Citations

Chavrier P., et al. (1992) Gene. 112:261-264.; ; Novick P., et al. (1993) Cell. 75:597-601.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.2M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002859

Uniprot Accession Number

P61020

Species

Human

AA Sequence

MTSRSTA RPNGQPQASK ICQFKLVLLG ESAVGKSSLV LRFVKGQFHE YQESTIGAAF LTQSVCLDDT TVKFEIWDTA GQERYHSLAP MYYRGAQAAI VVYDITNQET FARAKTWVKE LQRQASPSIV IALAGNKADL ANKRMVEYEE AQAYADDNSL LFMETSAKTA MNVNDLFLAI AKKLPKSEPQ NLGGAAGRSR GVDLHEQSQQ NKSQCCSN