Recombinant human RAB5B protein
Ras-related protein Rab-5B, also known as RAB5B, is a member of the Rab family of small (monomeric) G proteins. RAB5B is involved in endocytosis and recycling of cell surface molecules. It interacts with RIN2 and RIN3, which regulate its function, possibly by acting as GEFs. Knockdown of RAB5B abolished group I metabotropic glutamate receptor (mGluR) -mediated neuroprotection. Recombinant human RAB5B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ras-related protein Rab-5B, Rab 5b, Rab5 b, RAB5B member RAS oncogene family, Ras related protein Rab 5B
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.1 kDa (238aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chavrier P., et al. (1992) Gene. 112:261-264.; ; Novick P., et al. (1993) Cell. 75:597-601.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.2M NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002859
Uniprot Accession Number
P61020
Species
Human
AA Sequence
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