Recombinant human Thioredoxin Reductase 1/TXNRD1 (selenocys648cys) protein

TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. Recombinant human TXNRD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

Product Name Alternative

GRIM 12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, TR, TR 1, TRXR 1, TXNR, TXNRD 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 15unit/mg, and was measured in a coupled assay with 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.

Molecular Weight

55.9 kDa (510aa) confirmed by MALDI-TOF

Additionnal Information

GRIM 12, GRIM12, GRIM-12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, Thioredoxin reductase GRIM12, TR, TR 1, TR1, TR-1, TRXR 1, TRXR1, TRXR-1, TXNR, TXNRD 1, TXNRD1, TXNRD-1, ATGP0844-10 µg, ATGP0844-20 µg, ATGP0844-50 µg, ATGP0844-100 µg, ATGP0844-250 µg, ATGP0844-500 µg, ATGP0844-1 mg, ATGP0844-010, ATGP0844-020, ATGP0844-050, ATGP0844-100, ATGP0844-250, ATGP0844-500, ATGP0844-01M

References & Citations

Ma X., et al. (2002) J Biol Chem. 277 (25) :22460-8. ; ; Javvadi P., et al. (2010) Cancer Res. 70 (5) :1941-50.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1mM PMSF, 0.1M NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_001087240

Uniprot Accession Number

Q16881

Species

Human

AA Sequence

YDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGCCG