Recombinant human ISCU protein
Iron-sulfur cluster assembly enzyme, also known as ISCu, is a member of the nifu family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCu-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. Recombinant human ISCu protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
16.7 kDa (154aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Tong WH., et al. (2000) EMBO J. 19:5692-5700; ; Tong WH, et al. (2006) . Cell Metab. 3 (3) : 199-210.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_998760
Uniprot Accession Number
Q9H1K1
Species
Human
AA Sequence
YHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
