Recombinant human G3BP1 protein

G3BP1 is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. And it cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-uTR. Recombinant human G3BP1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

G3BP stress granule assembly factor 1, GTPase activating protein (SH3 domain) binding protein 1, Ras-GTPase-activating protein SH3-domain-binding protein, DNA helicase VIII, ATP-dependent DNA helicase VIII, GAP SH3 domain-binding protein 1, DH-VIII, G3BP, G3BP-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

53.2 kDa (474aa) confirmed by MALDI-TOF

Additionnal Information

Ras-GTPase-activating protein SH3-domain-binding protein, RasGAP associated endoribonuclease G3BP, Ras GTPase-activating protein-binding protein 1, Ras GTPase activating protein SH3 domain binding protein, Ras GTPase activating protein binding protein 1, MGC111040, HDH VIII, GTPase activating protein binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, GAP SH3 domain-binding protein 1, GAP SH3 domain binding protein 1, GAP binding protein, G3BP-1, G3BP1, G3BP stress granule assembly factor 1, G3BP 1, G3BP, DNA helicase VIII, DH-VIII, DHVIII, DH VIII, ATP-dependent DNA helicase VIII, ATP dependent DNA helicase VIII, ATGP0841-10 µg, ATGP0841-20 µg, ATGP0841-50 µg, ATGP0841-100 µg, ATGP0841-250 µg, ATGP0841-500 µg, ATGP0841-1 mg, ATGP0841-010, ATGP0841-020, ATGP0841-050, ATGP0841-100, ATGP0841-250, ATGP0841-500, ATGP0841-01M

References & Citations

Kurz, J.C., et al. (2000) Curr. Opin. Chem. Biol. 4: 553-558.; ; van Eenennaam, H., et al. (2001) Mol. Biol. Cell. 12: 3680-3689.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005745.1

Uniprot Accession Number

Q13283

Species

Human

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