Recombinant human G3BP1 protein
G3BP1 is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. And it cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-uTR. Recombinant human G3BP1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
53.2 kDa (474aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kurz, J.C., et al. (2000) Curr. Opin. Chem. Biol. 4: 553-558.; ; van Eenennaam, H., et al. (2001) Mol. Biol. Cell. 12: 3680-3689.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005745.1
Uniprot Accession Number
Q13283
Species
Human
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Lentiviral human DIS3L2 shRNA (UAS) - Lentiviral human DIS3L2 shRNA (UAS, GFP) plasmid
