Recombinant human RalB protein
RALB, also called Ras-related protein Ral-B, belongs to subfamily of Ras-related GTPases. This protein is activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. It plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Also it is required for suppression of apoptosis. Recombinant human RALB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Ras-related protein Ral-B, RAS like proto-oncogene B, Ras related GTP binding protein B
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
25.6 kDa (227aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559.; ; Jullien-Flores V., et al. (1995) . J. Biol. Chem. 270: 22473-22477
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002872
Uniprot Accession Number
P11234
Species
Human
AA Sequence
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