Recombinant human RalB protein

RALB, also called Ras-related protein Ral-B, belongs to subfamily of Ras-related GTPases. This protein is activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. It plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Also it is required for suppression of apoptosis. Recombinant human RALB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Ras-related protein Ral-B, RAS like proto-oncogene B, Ras related GTP binding protein B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.6 kDa (227aa) confirmed by MALDI-TOF

Additionnal Information

RalB, Ras-related protein Ral-B, Ras related protein RalB, Ras related protein Ral B, ATGP0839-10 µg, ATGP0839-20 µg, ATGP0839-50 µg, ATGP0839-100 µg, ATGP0839-250 µg, ATGP0839-500 µg, ATGP0839-1 mg, ATGP0839-010, ATGP0839-020, ATGP0839-050, ATGP0839-100, ATGP0839-250, ATGP0839-500, ATGP0839-01M

References & Citations

Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559.; ; Jullien-Flores V., et al. (1995) . J. Biol. Chem. 270: 22473-22477

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002872

Uniprot Accession Number

P11234

Species

Human

AA Sequence

HMAANKS KGQSSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLLVFSIT EHESFTATAE FREQILRVKA EEDKIPLLVV GNKSDLEERR QVPVEEARSK AEEWGVQYVE TSAKTRANVD KVFFDLMREI RTKKMSENKD KNGKKSSKNK KSFKERC