Recombinant human AUH protein
AUH, also known as mitochondrial methylglutaconyl-CoA hydratase, is involved in the amino acid degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water. It has been found to have very low enoyl-CoA hydratase activity. Recombinant human AUH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
31.4 kDa (293aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kurimoto K, et al. (2009) . Proteins 75 (2) :360-72; ; Mack M., et al. (2006) . FEBS J. 273 (9) :2012-22
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001689
Uniprot Accession Number
Q13825
Species
Human
AA Sequence
Frequently Asked Questions
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