Recombinant human HRSP12/RIDA protein

HRSP12, also called ribonuclease uK114, is an endoribonuclease found predominantly in human adult kidney and liver, and which is responsible for inhibiting translation by cleaving mRNA. This protein cleaves phosphodiester bonds only in single-stranded RNA. It may be an important biomarker for heptatic carcinoma. Recombinant human HRSP12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Reactive intermediate imine deaminase A homolog, 2-iminobutanoate/2-iminopropanoate deaminase, Heat-responsive protein 12, Translational inhibitor p14.5, Translation inhibitor L-PSP ribonuclease, UK114 antigen homolog, P14.5, PSP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.6 kDa (157aa) confirmed by MALDI-TOF

Additionnal Information

HRSP 12, Ribonuclease uK114, p14.5, PSP, uK114, ATGP0837-10 µg, ATGP0837-20 µg, ATGP0837-50 µg, ATGP0837-100 µg, ATGP0837-250 µg, ATGP0837-500 µg, ATGP0837-1 mg, ATGP0837-10, ATGP0837-20, ATGP0837-50, ATGP0837-100, ATGP0837-250, ATGP0837-500, ATGP0837-1

References & Citations

Schmiedeknecht G., et al. (1996) . Eur J Biochem. 242 (2) :339-51.; ; Manjasetty BA, et al. (2004) . Proteins 54 (4) :797-800

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_005827

Uniprot Accession Number

P52758

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL