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Recombinant human ROBLD3/LAMTOR2 protein

ROBLD3, also known as MAPBPIP, belongs to the GAMAD family. It is an adapter protein that enhances the efficiency of the MAP kinase cascade and facilitates the activation of MAPK2. ROBLD3 compels the recruitment of MP1 to late endosomes where they form a very stable heterodimeric complex required for ERK activation on endosomes. Recombinant human ROBLD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ENDAP, Endosomal adaptor protein p14, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, MAPBP-interacting protein, MAPBPIP, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen activated protein binding protein interacting protein, p14, Ragulator complex protein LAMTOR2, Ragulator2, Roadblock domain containing 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA)

Purity

> 95% by SDS-PAGE

Molecular Weight

16 kDa (149aa) confirmed by MALDI-TOF

Additionnal Information

ENDAP, Endosomal adaptor protein p14, HSPC003, LAMTOR2, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, MAPBP-interacting protein, MAPBPIP, MAPKSP1 adaptor protein, MAPKSP1AP, Mitogen activated protein binding protein interacting protein, Mitogen-activated protein-binding protein-interacting protein, p14, Ragulator complex protein LAMTOR2, Ragulator2, Roadblock domain containing 3, ROBLD3, RP11-336K24.9, ATGP0834-10 µg, ATGP0834-20 µg, ATGP0834-50 µg, ATGP0834-100 µg, ATGP0834-250 µg, ATGP0834-500 µg, ATGP0834-1 mg, ATGP0834-010, ATGP0834-020, ATGP0834-050, ATGP0834-100, ATGP0834-250, ATGP0834-500, ATGP0834-01M

References & Citations

Sancak Y., et al. (2010) Cell. 141 (2) :290-303.; ; Waters S., et al. (2009) FEBS Lett. 583 (12) :1846-52.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_054736.1

Uniprot Accession Number

Q9Y2Q5

Species

Human

AA Sequence

MLRPKA LTQVLSQANT GGVQSTLLLN NEGSLLAYSG YGDTDARVTA AIASNIWAAY DRNGNQAFNE DNLKFILMDC MEGRVAITRV ANLLLCMYAK ETVGFGMLKA KAQALVQYLE EPLTQVAAS

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