Recombinant human PTRH2 protein
PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1, is a mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondria to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. The natural substrate for PTRH2 may be petidyl-tRNAs which drop off the ribosome during protein synthesis. Recombinant human PTRH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Peptidyl-tRNA hydrolase 2, BIT1, PTH2, CGI-147, Bcl-2 inhibitor of transcription 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
14.9 kDa (137aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jan Y., et al. (2004) Cell. 116 (5) :751-62.; ; De Pereda JM., et al. (2004) J Biol Chem. 279 (9) :8111-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Apoptosis
NCBI Accession Number
NP_057161
Uniprot Accession Number
Q9Y3E5
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MEYKMILVVR NDLKMGKGKV AAQCSHAAVS AYKQIQRRNP EMLKQWEYCG QPKVVVKAPD EETLIALLAH AKMLGLTVSL IQDAGRTQIA PGSQTVLGIG PGPADLIDKV TGHLKLY
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