Recombinant human PTRH2 protein

PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1, is a mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondria to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. The natural substrate for PTRH2 may be petidyl-tRNAs which drop off the ribosome during protein synthesis. Recombinant human PTRH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Peptidyl-tRNA hydrolase 2, BIT1, PTH2, CGI-147, Bcl-2 inhibitor of transcription 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

14.9 kDa (137aa) confirmed by MALDI-TOF

Additionnal Information

PTRH2, Peptidyl-tRNA hydrolase 2, BIT1, PTH2, CGI-147, Bcl-2 inhibitor of transcription 1, ATGP0830-10 µg, ATGP0830-20 µg, ATGP0830-50 µg, ATGP0830-100 µg, ATGP0830-250 µg, ATGP0830-500 µg, ATGP0830-1 mg, ATGP0830-10, ATGP0830-20, ATGP0830-50, ATGP0830-100, ATGP0830-250, ATGP0830-500, ATGP0830-1

References & Citations

Jan Y., et al. (2004) Cell. 116 (5) :751-62.; ; De Pereda JM., et al. (2004) J Biol Chem. 279 (9) :8111-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_057161

Uniprot Accession Number

Q9Y3E5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEYKMILVVR NDLKMGKGKV AAQCSHAAVS AYKQIQRRNP EMLKQWEYCG QPKVVVKAPD EETLIALLAH AKMLGLTVSL IQDAGRTQIA PGSQTVLGIG PGPADLIDKV TGHLKLY