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Recombinant human ERP27 protein

ERP27 (endoplasmic reticulum protein 27 kDa) is widely expressed protein that localize to the ER and may act as proteases, protein disulfide isomerases, thiol-disulfide oxidases or phospholipases. ERP27 does not contain a CXXC active site motif indicating that it is a catalytically redox-inactive member of the protein disulfide isomerase family. Recombinant human ERP27 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Endoplasmic reticulum resident protein 27, PDIA8, C12orf46

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30 kDa (269aa) confirmed by MALDI-TOF

Additionnal Information

ERP27, Endoplasmic reticulum resident protein 27, PDIA8, C12orf46, ATGP0829-10 µg, ATGP0829-20 µg, ATGP0829-50 µg, ATGP0829-100 µg, ATGP0829-250 µg, ATGP0829-500 µg, ATGP0829-1 mg, ATGP0829-10, ATGP0829-20, ATGP0829-50, ATGP0829-100, ATGP0829-250, ATGP0829-500, ATGP0829-1

References & Citations

Alanen HI., et al. (2006) J Biol Chem. 281 (44) :33727-38. ; ; Alanen HI., et al. (2003) J Biol Chem. 278 (31) :28912-20.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_689534

Uniprot Accession Number

Q96DN0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEVEKSSDGP GAAQEPTWLT DVPAAMEFIA ATEVAVIGFF QDLEIPAVPI LHSMVQKFPG VSFGISTDSE VLTHYNITGN TICLFRLVDN EQLNLEDEDI ESIDATKLSR FIEINSLHMV TEYNPVTVIG LFNSVIQIHL LLIMNKASPE YEENMHRYQK AAKLFQGKIL FILVDSGMKE NGKVISFFKL KESQLPALAI YQTLDDEWDT LPTAEVSVEH VQNFCDGFLS GKLLKENRES EGKTPKVEL
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