Recombinant human Ribokinase/RBKS protein

Ribokinase, also known as RBKS, belongs to the pfkB family of carbohydrate kinases. It phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. Recombinant human RBKS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribokinase, RBSK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.3 kDa (342aa) confirmed by MALDI-TOF

Additionnal Information

RBKS, ATGP0827-10 µg, ATGP0827-20 µg, ATGP0827-50 µg, ATGP0827-100 µg, ATGP0827-250 µg, ATGP0827-500 µg, ATGP0827-1 mg, ATGP0827-10, ATGP0827-20, ATGP0827-50, ATGP0827-100, ATGP0827-250, ATGP0827-500, ATGP0827-1

References & Citations

Sigrell J A., et al. (1999) J Mol Biol. 290:1009-1018.; ; Park J., et al. (2007) FEBS Lett. 581:3211-3216.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing,10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_071411

Uniprot Accession Number

Q9H477

Species

Human

AA Sequence

MAASGEPQRQ WQEEVAAVVV VGSCMTDLVS LTSRLPKTGE TIHGHKFFIG FGGKGANQCV QAARLGAMTS MVCKVGKDSF GNDYIENLKQ NDISTEFTYQ TKDAATGTAS IIVNNEGQNI IVIVAGANLL LNTEDLRAAA NVISRAKVMV CQLEITPATS LEALTMARRS GVKTLFNPAP AIADLDPQFY TLSDVFCCNE SEAEILTGLT VGSAADAGEA ALVLLKRGCQ VVIITLGAEG CVVLSQTEPE PKHIPTEKVK AVDTTGAGDS FVGALAFYLA YYPNLSLEDM LNRSNFIAAV SVQAAGTQSS YPYKKDLPLT LF