Recombinant human PITPNA protein

Phosphatidylinositol transfer protein alpha, also known PITPNA, is found in the cytoplasm, where it catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes. PITPNA is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) by phosphoinositide-3-kinase. Recombinant human PITPNA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphatidylinositol transfer protein alpha isoform, PI-TP alpha, PITPN, VIB1A, PtdInsTP alpha

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33.9 kDa (290aa) confirmed by MALDI-TOF

Additionnal Information

PITPNA, Phosphatidylinositol transfer protein alpha isoform, PI-TP alpha, PITPN, VIB1A, PtdInsTP alpha, ATGP0826-10 µg, ATGP0826-20 µg, ATGP0826-50 µg, ATGP0826-100 µg, ATGP0826-250 µg, ATGP0826-500 µg, ATGP0826-1 mg, ATGP0826-10, ATGP0826-20, ATGP0826-50, ATGP0826-100, ATGP0826-250, ATGP0826-500, ATGP0826-1

References & Citations

Monaco M E., et al. (1998) Biochem J. 335:175-179.; ; Aikawa Y., et al. (1999) J Biol Chem. 274:20569-20577.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 1mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_006215

Uniprot Accession Number

Q00169

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MVLLKEYRVI LPVSVDEYQV GQLYSVAEAS KNETGGGEGV EVLVNEPYEK DGEKGQYTHK IYHLQSKVPT FVRMLAPEGA LNIHEKAWNA YPYCRTVITN EYMKEDFLIK IETWHKPDLG TQENVHKLEP EAWKHVEAVY IDIADRSQVL SKDYKAEEDP AKFKSIKTGR GPLGPNWKQE LVNQKDCPYM CAYKLVTVKF KWWGLQNKVE NFIHKQERRL FTNFHRQLFC WLDKWVDLTM DDIRRMEEET KRQLDEMRQK DPVKGMTADD