Recombinant human CDKN3 protein

Cyclin-dependent kinase inhibitor 3, also known CDKN3, belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. Also CDKN3 is important in cell cycle regulation. Recombinant human CDKN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase inhibitor 3, CDI1, CIP2, KAP, KAP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.9 kDa (232aa) confirmed by MALDI-TOF

Additionnal Information

CDKN3, Cyclin-dependent kinase inhibitor 3, CDI1, CIP2, KAP, KAP1, ATGP0825-10 µg, ATGP0825-20 µg, ATGP0825-50 µg, ATGP0825-100 µg, ATGP0825-250 µg, ATGP0825-500 µg, ATGP0825-1 mg, ATGP0825-10, ATGP0825-20, ATGP0825-50, ATGP0825-100, ATGP0825-250, ATGP0825-500, ATGP0825-1

References & Citations

Maak S., et al. (2003) Genet Sel Evol. 1:157-165.; ; Gyuris J., et al. (1993) Cell. 75:791-803.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_005183.2

Uniprot Accession Number

Q16667

Species

Human

AA Sequence

MKPPSSIQTS EFDSSDEEPI EDEQTPIHIS WLSLSRVNCS QFLGLCALPG CKFKDVRRNV QKDTEELKSC GIQDIFVFCT RGELSKYRVP NLLDLYQQCG IITHHHPIAD GGTPDIASCC EIMEELTTCL KNYRKTLIHC YGGLGRSCLV AACLLLYLSD TISPEQAIDS LRDLRGSGAI QTIKQYNYLH EFRDKLAAHL SSRDSQSRSV SR