Recombinant human MDP1 protein

MDP1 (Magnesium-dependent phosphatase 1) belongs to the HAD-like hydrolase superfamily. This protein is a magnesium-dependent phosphatase which may act as a tyrosine phosphatase. It is inhibited by vanadate and zinc, and slightly by calcium. Recombinant human MDP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Magnesium-dependent phosphatase 1, MDP-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.6 kDa (200aa) confirmed by MALDI-TOF

Additionnal Information

MDP1, Magnesium-dependent phosphatase 1, MDP-1, ATGP0823-10 µg, ATGP0823-20 µg, ATGP0823-50 µg, ATGP0823-100 µg, ATGP0823-250 µg, ATGP0823-500 µg, ATGP0823-1 mg, ATGP0823-10, ATGP0823-20, ATGP0823-50, ATGP0823-100, ATGP0823-250, ATGP0823-500, ATGP0823-1

References & Citations

Fortpied J., et al. (2006) J Biol Chem. 281 (27) :18378-85.; ; Selengut JD., et al. (2000) Biochemistry 39 (28) :8315-24.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_612485

Uniprot Accession Number

Q86V88

Species

Human

AA Sequence

MARLPK LAVFDLDYTL WPFWVDTHVD PPFHKSSDGT VRDRRGQDVR LYPEVPEVLK RLQSLGVPGA AASRTSEIEG ANQLLELFDL FRYFVHREIY PGSKITHFER LQQKTGIPFS QMIFFDDERR NIVDVSKLGV TCIHIQNGMN LQTLSQGLET FAKAQTGPLR SSLEESPFEA