Recombinant human RalA protein

RALA (Ras-related protein Ral-A) belongs to the small GTPase superfamily, Ras family of proteins. RALA mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. RALA is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis. Recombinant human RALA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Ral-A, RAL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.8 kDa (227aa) confirmed by MALDI-TOF

Additionnal Information

RalA, Ras-related protein Ral-A, Ras related protein RalA, Ras related protein Ral A, ATGP0822-10 µg, ATGP0822-20 µg, ATGP0822-50 µg, ATGP0822-100 µg, ATGP0822-250 µg, ATGP0822-500 µg, ATGP0822-1 mg, ATGP0822-010, ATGP0822-020, ATGP0822-050, ATGP0822-100, ATGP0822-250, ATGP0822-500, ATGP0822-01M

References & Citations

Cascone I., et al. (2008) EMBO J. 27:2375-2387; ; Balasubramanian N., et al. (2009) Curr. Biol. 20:75-79

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl, 0.1mM PMSF

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005393

Uniprot Accession Number

P11233

Species

Human

AA Sequence

MAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA EQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC