Recombinant human RPP30 protein
RPP30 (Ribonuclease P protein subunit p30) belongs to the eukaryotic/archaeal RNase P protein component 3 family. RPP30 is component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P (PNase P) is small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. Also, RNase P accumulates in the nucleolus, have a similar RNA component and also have several protein subunits in common. Recombinant human RPP30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ribonuclease P protein subunit p30, RNASEP2, TSG15
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
31.8 kDa (291aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kurz, J.C., et al. (2000) Curr. Opin. Chem. Biol. 4: 553-558.; ; van Eenennaam, H., et al. (2001) Mol. Biol. Cell. 12: 3680-3689.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl, 1mM EDTA
Scientific Category
Metabolism
NCBI Accession Number
NP_006404
Uniprot Accession Number
P78346
Species
Human
AA Sequence
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