Recombinant human RPP30 protein

RPP30 (Ribonuclease P protein subunit p30) belongs to the eukaryotic/archaeal RNase P protein component 3 family. RPP30 is component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P (PNase P) is small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. Also, RNase P accumulates in the nucleolus, have a similar RNA component and also have several protein subunits in common. Recombinant human RPP30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribonuclease P protein subunit p30, RNASEP2, TSG15

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.8 kDa (291aa) confirmed by MALDI-TOF

Additionnal Information

RPP30, Ribonuclease P protein subunit p30, RNASEP2, TSG15, ATGP0821-10 µg, ATGP0821-20 µg, ATGP0821-50 µg, ATGP0821-100 µg, ATGP0821-250 µg, ATGP0821-500 µg, ATGP0821-1 mg, ATGP0821-10, ATGP0821-20, ATGP0821-50, ATGP0821-100, ATGP0821-250, ATGP0821-500, ATGP0821-1

References & Citations

Kurz, J.C., et al. (2000) Curr. Opin. Chem. Biol. 4: 553-558.; ; van Eenennaam, H., et al. (2001) Mol. Biol. Cell. 12: 3680-3689.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl, 1mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_006404

Uniprot Accession Number

P78346

Species

Human

AA Sequence

MAVFADL DLRAGSDLKA LRGLVETAAH LGYSVVAINH IVDFKEKKQE IEKPVAVSEL FTTLPIVQGK SRPIKILTRL TIIVSDPSHC NVLRATSSRA RLYDVVAVFP KTEKLFHIAC THLDVDLVCI TVTEKLPFYF KRPPINVAID RGLAFELVYS PAIKDSTMRR YTISSALNLM QICKGKNVII SSAAERPLEI RGPYDVANLG LLFGLSESDA KAAVSTNCRA ALLHGETRKT AFGIISTVKK PRPSEGDEDC LPASKKAKCE G