Recombinant human HDHD2 protein

Haloacid dehalogenase-like hydrolase domain containing 2, also known HDHD2, belongs to the HAD-like hydrolase superfamily. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. HDHD2 has two active sites, an L-2-haloacid dehalogenase and a carboxylate group. The L-2-haloacid dehalogenase active site catalyzes the hydrolytic dehalogenation of D- and L-2-haloalkanoic acids, producing L- and D-2-hydroxyalkanoic acids. Recombinant human HDHD2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Haloacid dehalogenase-like hydrolase domain containing 2, 3110052N05Rik, DKFZp564D1378

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.6 kDa (279aa) confirmed by MALDI-TOF

Additionnal Information

HDHD2, Haloacid dehalogenase-like hydrolase domain containing 2, 3110052N05Rik, DKFZp564D1378, ATGP0818-10 µg, ATGP0818-20 µg, ATGP0818-50 µg, ATGP0818-100 µg, ATGP0818-250 µg, ATGP0818-500 µg, ATGP0818-1 mg, ATGP0818-10, ATGP0818-20, ATGP0818-50, ATGP0818-100, ATGP0818-250, ATGP0818-500, ATGP0818-1

References & Citations

Carstea E D., et al. (1993) Proc Matl Acad Sci uSA. 90:2002-2004.; ; Beiraqhi S., et al. (2007) Am J Hum Genet. 81:180-188.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_115500

Uniprot Accession Number

Q9H0R4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAACRALKAV LVDLSGTLHI EDAAVPGAQE ALKRLRGASV IIRFVTNTTK ESKQDLLERL RKLEFDISED EIFTSLTAAR SLLERKQVRP MLLVDDRALP DFKGIQTSDP NAVVMGLAPE HFHYQILNQA FRLLLDGAPL IAIHKARYYK RKDGLALGPG PFVTALEYAT DTKATVVGKP EKTFFLEALR GTGCEPEEAV MIGDDCRDDV GGAQDVGMLG ILVKTGKYRA SDEEKINPPP YLTCESFPHA VDHILQHLL