Recombinant human HDHD2 protein
Haloacid dehalogenase-like hydrolase domain containing 2, also known HDHD2, belongs to the HAD-like hydrolase superfamily. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. HDHD2 has two active sites, an L-2-haloacid dehalogenase and a carboxylate group. The L-2-haloacid dehalogenase active site catalyzes the hydrolytic dehalogenation of D- and L-2-haloalkanoic acids, producing L- and D-2-hydroxyalkanoic acids. Recombinant human HDHD2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Haloacid dehalogenase-like hydrolase domain containing 2, 3110052N05Rik, DKFZp564D1378
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.6 kDa (279aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Carstea E D., et al. (1993) Proc Matl Acad Sci uSA. 90:2002-2004.; ; Beiraqhi S., et al. (2007) Am J Hum Genet. 81:180-188.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_115500
Uniprot Accession Number
Q9H0R4
Species
Human
AA Sequence
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