Recombinant human GNPNAT1 protein

Glucosamine-phosphate N-acetyltransferase, also known GNPNAT1, belongs to the GNA1 subfamily of the larger acetyltransferase family of proteins. It is localized to the Golgi apparatus and the endosome. It is important for uDPGlcNAc biosynthesis pathway. GNPNAT1 catalyzes the synthesis of GlcNAc6P from AcCoA and GlcN6P, a step in the uDP-GlcNAc6P formation pathway. Recombinant human GNPNAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glucosamine-phosphate N-acetyltransferase, GNPNAT, Gpnat1, Phosphoglucosamine acetylase, Phosphoglucosamine transacetylase, GNA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.1 kDa (207aa) confirmed by MALDI-TOF

Additionnal Information

GNPNAT1, Glucosamine-phosphate N-acetyltransferase, GNPNAT, Gpnat1, ATGP0817-10 µg, ATGP0817-20 µg, ATGP0817-50 µg, ATGP0817-100 µg, ATGP0817-250 µg, ATGP0817-500 µg, ATGP0817-1 mg, ATGP0817-10, ATGP0817-20, ATGP0817-50, ATGP0817-100, ATGP0817-250, ATGP0817-500, ATGP0817-1

References & Citations

Jiang, H., et al. (2005) Plant Physiol. 138:232-242.; ; Mio T., et al. (2007) Microbiology. 146:1753-1758.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_932332

Uniprot Accession Number

Q96EK6

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMKPDETP MFDPSLLKEV DWSQNTATFS PAISPTHPGE GLVLRPLCTA DLNRGFFKVL GQLTETGVVS PEQFMKSFEH MKKSGDYYVT VVEDVTLGQI VATATLIIEH KFIHSCAKRG RVEDVVVSDE CRGKQLGKLL LSTLTLLSKK LNCYKITLEC LPQNVGFYKK FGYTVSEENY MCRRFLK