Recombinant human GNPNAT1 protein
Glucosamine-phosphate N-acetyltransferase, also known GNPNAT1, belongs to the GNA1 subfamily of the larger acetyltransferase family of proteins. It is localized to the Golgi apparatus and the endosome. It is important for uDPGlcNAc biosynthesis pathway. GNPNAT1 catalyzes the synthesis of GlcNAc6P from AcCoA and GlcN6P, a step in the uDP-GlcNAc6P formation pathway. Recombinant human GNPNAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glucosamine-phosphate N-acetyltransferase, GNPNAT, Gpnat1, Phosphoglucosamine acetylase, Phosphoglucosamine transacetylase, GNA1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.1 kDa (207aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jiang, H., et al. (2005) Plant Physiol. 138:232-242.; ; Mio T., et al. (2007) Microbiology. 146:1753-1758.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_932332
Uniprot Accession Number
Q96EK6
Species
Human
AA Sequence
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