Recombinant human ARL5/ARL5A protein
ADP-ribosylation factor-like 5A, also known ARL5A, belongs to the ARF family of GTP-binding proteins. ARFs are important in eukaryotic vesicular trafficking pathways and play an essential role in the activation of phospholipase D. unlike many ARF family members, ARL5 is thought to lack ADP-ribosylation enhancing activity. ARL5A is developmentally regulated and may play a role in nuclear dynamics and signaling cascades during embryonic development. Recombinant human ARL5A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
ADP-ribosylation factor-like 5A, ARFLP5, ARL5, ADP ribosylation factor like GTPase 5A, ADP-ribosylation factor-like 5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.3 kDa (203aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Haraquchi T., et al. (2006) Biosci Biotechnol Biochem. 70:1798-1802.; ; Pasqualato S., et al. (2002) EMBO Rep. 3:1035-1041.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF
Scientific Category
Signal Transduction
NCBI Accession Number
NP_036229.1
Uniprot Accession Number
Q9Y689
Species
Human
AA Sequence
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