Recombinant human HUS1 protein
HuS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1 (RAD9-RAD1-HuS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. Recombinant human HuS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Checkpoint protein HuS1, HUS1 checkpoint clamp component, HUS1 checkpoint homolog, hus1+-like protein, hHUS1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
33.8 kDa (300aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Weiss RS., et al (2009) Proc Natl Acad Sci u S A. 106 (50) :21282-7.; ; Roos-Mattjus P., et al (2002) J Biol Chem. 15; ;277 (46) :43809-12.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 40% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004498
Uniprot Accession Number
O60921
Species
Human
AA Sequence
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