Recombinant human HUS1 protein

HuS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1 (RAD9-RAD1-HuS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. Recombinant human HuS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Checkpoint protein HuS1, HUS1 checkpoint clamp component, HUS1 checkpoint homolog, hus1+-like protein, hHUS1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33.8 kDa (300aa) confirmed by MALDI-TOF

Additionnal Information

HuS1, checkpoint protein HuS1, ATGP0815-10 µg, ATGP0815-20 µg, ATGP0815-50 µg, ATGP0815-100 µg, ATGP0815-250 µg, ATGP0815-500 µg, ATGP0815-1 mg, ATGP0815-10, ATGP0815-20, ATGP0815-50, ATGP0815-100, ATGP0815-250, ATGP0815-500, ATGP0815-1

References & Citations

Weiss RS., et al (2009) Proc Natl Acad Sci u S A. 106 (50) :21282-7.; ; Roos-Mattjus P., et al (2002) J Biol Chem. 15; ;277 (46) :43809-12.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 40% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004498

Uniprot Accession Number

O60921

Species

Human

AA Sequence

MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS