Recombinant human NHP2L1 protein
NHP2L1 belongings to the ribosomal protein L7Ae family. This protein localizes to the nucleus, mainly concentrated in the dense fibrillar component of the nucleolus. It directly binds to the 5' stem-loop of u4 snRNA and plays an important role in the late stage of spliceosome assembly, prior to step I of splicing catalysis. Recombinant human NHP2L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
NHP2 (non-histone chromosome protein 2) -like 1, FA1, NHPX, OTK27, SNRNP15-5, SNu13, SPAG12, SSFA1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
16.7 kDa (152aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Luhrmann R., et al. (2006) Mol Cell Biol. 26 (13) :5146-54.; ; Jovin TM., et al (2005) RNA. 11 (2) :197-209.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004999
Uniprot Accession Number
P55769
Species
Human
AA Sequence
Frequently Asked Questions
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