Recombinant human NHP2L1 protein

NHP2L1 belongings to the ribosomal protein L7Ae family. This protein localizes to the nucleus, mainly concentrated in the dense fibrillar component of the nucleolus. It directly binds to the 5' stem-loop of u4 snRNA and plays an important role in the late stage of spliceosome assembly, prior to step I of splicing catalysis. Recombinant human NHP2L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

NHP2 (non-histone chromosome protein 2) -like 1, FA1, NHPX, OTK27, SNRNP15-5, SNu13, SPAG12, SSFA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.7 kDa (152aa) confirmed by MALDI-TOF

Additionnal Information

NHP2L1, NHP2 (non-histone chromosome protein 2) -like 1, FA1, NHPX, OTK27, SNRNP15-5, SNu13, SPAG12, SSFA1, ATGP0812-10 µg, ATGP0812-20 µg, ATGP0812-50 µg, ATGP0812-100 µg, ATGP0812-250 µg, ATGP0812-500 µg, ATGP0812-1 mg, ATGP0812-10, ATGP0812-20, ATGP0812-50, ATGP0812-100, ATGP0812-250, ATGP0812-500, ATGP0812-1

References & Citations

Luhrmann R., et al. (2006) Mol Cell Biol. 26 (13) :5146-54.; ; Jovin TM., et al (2005) RNA. 11 (2) :197-209.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004999

Uniprot Accession Number

P55769

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV