Recombinant human PYCR1 protein
PYCR1 is a universal housekeeping enzyme that catalyzes the NAD (P) H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP (+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B) . Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
35.5 kDa (339aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kornak u., et al. (2009) Nat Genet. 41 (9) :1016-21.; ; Rao Z., et al (2006) J Mol Biol. 359 (5) :1364-77.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_008838
Uniprot Accession Number
P32322
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband®, Cy3 Conjugate
