Recombinant human PYCR1 protein

PYCR1 is a universal housekeeping enzyme that catalyzes the NAD (P) H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP (+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B) . Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C.

Product Name Alternative

Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C.

Molecular Weight

35.5 kDa (339aa) confirmed by MALDI-TOF

Additionnal Information

PYCR1, Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3, ATGP0811-10 µg, ATGP0811-20 µg, ATGP0811-50 µg, ATGP0811-100 µg, ATGP0811-250 µg, ATGP0811-500 µg, ATGP0811-1 mg, ATGP0811-10, ATGP0811-20, ATGP0811-50, ATGP0811-100, ATGP0811-250, ATGP0811-500, ATGP0811-1

References & Citations

Kornak u., et al. (2009) Nat Genet. 41 (9) :1016-21.; ; Rao Z., et al (2006) J Mol Biol. 359 (5) :1364-77.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_008838

Uniprot Accession Number

P32322

Species

Human

AA Sequence

MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD