Recombinant human Glyoxalase II protein

HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 500 units/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.

Product Name Alternative

Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 500unit/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.

Molecular Weight

31.4 kDa (284aa) confirmed by MALDI-TOF

Additionnal Information

Glyoxalase II, Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1, ATGP0808-10 µg, ATGP0808-20 µg, ATGP0808-50 µg, ATGP0808-100 µg, ATGP0808-250 µg, ATGP0808-500 µg, ATGP0808-1 mg, ATGP0808-10, ATGP0808-20, ATGP0808-50, ATGP0808-100, ATGP0808-250, ATGP0808-500, ATGP0808-1

References & Citations

Vila AJ., et al. (2010) J Inorg Biochem. 104 (7) :726-31.; ; Crowder MW., et al (2009) Biochemistry. 48 (23) :5426-34.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001035517

Uniprot Accession Number

Q16775

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD