Recombinant human SMNDC1 protein

SMNDC1 (survival motor neuron domain containing 1) is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (Survival Motor Neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. Mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. Recombinant human SMNDC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Survival of motor neuron-related-splicing factor 30, Survival of motor neuron-related-splicing factor 30

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.9 kDa (258aa) confirmed by MALDI-TOF

Additionnal Information

SMNDC1, Survival of motor neuron-related-splicing factor 30, Survival of motor neuron-related-splicing factor 30, ATGP0807-10 µg, ATGP0807-20 µg, ATGP0807-50 µg, ATGP0807-100 µg, ATGP0807-250 µg, ATGP0807-500 µg, ATGP0807-1 mg, ATGP0807-10, ATGP0807-20, ATGP0807-50, ATGP0807-100, ATGP0807-250, ATGP0807-500, ATGP0807-1

References & Citations

Meister G., et al. (2001) EMBO J. 20:2304-2314; ; Talbot K., et al. (1998) Hum. Mol. Genet. 7:2149-2156

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Scientific Category

Apoptosis

NCBI Accession Number

NP_005862

Uniprot Accession Number

O75940

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSEDLAKQLA SYKAQLQQVE AALSGNGENE DLLKLKKDLQ EVIELTKDLL STQPSETLAS SDSFASTQPT HSWKVGDKCM AVWSEDGQCY EAEIEEIDEE NGTAAITFAG YGNAEVTPLL NLKPVEEGRK AKEDSGNKPM SKKEMIAQQR EYKKKKALKK AQRIKELEQE REDQKVKWQQ FNNRAYSKNK KGQVKRSIFA SPESVTGKVG VGTCGIADKP MTQYQDTSKY NVRHLMPQ