Recombinant human ARF6 protein

ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. Recombinant human ARF6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ADP-ribosylation factor 6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.2 kDa (195aa) confirmed by MALDI-TOF

Additionnal Information

ARF6, ATGP0802-10 µg, ATGP0802-20 µg, ATGP0802-50 µg, ATGP0802-100 µg, ATGP0802-250 µg, ATGP0802-500 µg, ATGP0802-1 mg, ATGP0802-10, ATGP0802-20, ATGP0802-50, ATGP0802-100, ATGP0802-250, ATGP0802-500, ATGP0802-1

References & Citations

Amor J C., et al. (1994) Nature. 372:704-708.; ; Randazzo P A., et al. (1994) J Biol Chem. 269:29490-29494.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001654

Uniprot Accession Number

P62330

Species

Human

AA Sequence

MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD GLYEGLTWLT SNYKS